Molecules and Cells

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Fig. 2.

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Fig. 2. Screening calmodulin-binding regions to produce efficient luciferase activities. Combinations of SmBiT-tagged calmodulin proteins at the N- or C-terminal, and LgBiT-tagged MYLK1S (A), MYLK2S (B), and CAMK2S (C) at the N- or C-terminal were coexpressed with 5-HT2AR in HEK293 cells. 5-HT-induced luciferase activities were measured as described in the Materials and Methods section. Veh., vehicle. (D) Dose dependency of 5-HT-stimulated maximum luciferase activities in cells expressing 5-HT2AR and CM-SmBiT with LgBiT-MYLK1S or LgBiT-MYLK2S. Also see Supplementary Figs. S2 and S3. Data were presented as mean ± SD. All results represent more than three different experiments.
Mol. Cells 2020;43:909~920
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