Fig. 5. Dendritic ATX2 toxicity requires its interactions with co-factors through the PAM2 motif . (A) Representative images of dendrites of C4da neurons of Control or expressing denoted transgenes (Genotype: Control, +/+; ppk^1a> UAS-CD4-tdTom/UAS-luciferase; PABP, +/+; ppk^1a> UAS-CD4-tdTom/UAS-PABP 3xHA; ATX2, UAS-ATX2/+; ppk^1a> UAS-CD4-tdTom/UAS-luciferase; PABP + ATX2, UAS-ATX2; ppk^1a> UAS-CD4-tdTom/UAS-PABP 3xHA). Scale bar = 100 µm. (B) Quantification of dendritic receptive fields of neurons expressing denoted transgenes described in Fig. 5A. (C) Representative images of FMR1-GFP (cyan) in da neuron clusters (magenta) expressing denoted transgene (Genotype: Control, 109(2)80-Gal4 > UAS-mCD2-RFP/+; UAS-FMR1-GFP/UAS-luciferase; ATX2, 109(2)80-Gal4 > UAS-mCD2-RFP/UAS-ATX2; UAS-FMR1-GFP/+; ATX2-ΔPAM2, 109(2)80-Gal4 > UAS-mCD2-RFP/+; UAS-FMR1-GFP/UAS-atx2 △ClA3). Scale bar = 20 µm. White arrowheads refer to FMR1-GFP in somatic areas while yellow arrowheads refer to dendritic FMR1-GFP punctae. (D) Quantification of the average FMR1-GFP intensity in somatic C4da neurons expressing denoted transgenes described in Fig. 5C. *P < 0.05 by one-way ANOVA with Tukey’s post-hoc correction. n ≥ 9 neurons. (E) Quantification of the number of FMR1-GFP puncta in dendritic areas in C4da neurons expressing denoted transgenes described in Fig. 5C. *P < 0.05, **P < 0.01 by one-way ANOVA with Tukey’s post-hoc correction. n ≥ 9 neurons.

Mol. Cells 2020;43:870~879 https://doi.org/10.14348/molcells.2020.0158

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