Fig. 4. Decrease in FMRP level is a neuronal compensatory response to the aberrant upregulation of ATX2. (A) Representative images of dendrites of C4da neurons of Control or expressing denoted transgenes (Genotype: Control,
+/+; ppk1a>
UAS-CD4-tdTom/UAS-luciferase; FMRP,
+/+; ppk1a>
UAS-CD4-tdTom/UAS-FMR1; FMRP RNAi,
+/+; ppk1a>
UAS-CD4-tdTom/UAS-FMRP RNAi, ATX2,
UAS-ATX2/+; ppk1a>
UAS-CD4-tdTom/UAS-luciferase; ATX2 + FMRP,
UAS-ATX2/+; ppk1a>
UAS-CD4-tdTom/UAS-FMR1; ATX2 + FMRP RNAi,
UAS-ATX2/+; ppk1a>
UAS-CD4-tdTom/UAS-FMRP RNAi). Scale bar = 100 µm. (B) Quantification of dendritic receptive fields of neurons expressing denoted transgenes described in
Fig. 4A. Gray-dotted line indicates the differences in group II and III catagorized dendrite proportions of ATX2 and ATX2 + FMRP RNAi expressing C4da neurons. (C) A sholl analysis of C4da neuron dendrites in 3rd instar larvae expressing the denoted transgenes (Genotype: ATX2,
UAS-ATX2/+; ppk1a>
UAS-CD4-tdTom/UAS-luciferase; ATX2 + FMRP RNAi,
UAS-ATX2/+; ppk1a>
UAS-CD4-tdTom/UAS-FMRP RNAi). *
P < 0.05 by two-tailed
t-test; error bars ± SEM; n = 9 replicates.
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