Fig. 1. ATX2 is associated with the regulation of both dendritic terminals and receptive field coverage in Drosophila C4da neurons. (A) Representative images of dendrites of C4da neurons of Control or expressing denoted ATX2 transgenes (Genotype: Control, +/+; ppk^1a> UAS-CD4-tdTom/UAS-luciferase; ATX2, UAS-ATX2/+; ppk^1a> UAS-CD4-tdTom/+; ATX2-NLS, UAS-flag-ATX2 NLS/+; ppk^1a> UAS-CD4-tdTom/+). Scale bar = 100 µm. (B) A schematic image illustrating how dendrites are grouped into similar level of dendritic receptive fields. Below are representative images of ATX2-induced dendritic phenotypes and their corresponding groups. (C) Quantification of dendritic receptive fields of neurons expressing denoted ATX2 transgenes described in Fig. 1A. (D) Representative image of dendrites of C4da neurons with ATX2 knockout genes (Genotype: ATX2^–/–, 109-Gal4 > SOP-FLP, UAS-mCD8GFP/+; FRT82B, atx2X1/FRT82B, tub-Gal80). Scale bar = 20 µm. (E) Quantification of dendritic receptive fields of neurons expressing denoted ATX2 transgenes described in Fig. 1D and (Genotype: Control, +/+; ppk^1a> UAS-CD4-tdTom/UAS-luciferase). (F) Quantification of the dendritic branches of neurons expressing denoted ATX2 transgenes described in Figs. 1D and 1E. **P < 0.01, ***P < 0.01 by two-tailed t-test; error bar ± SEM; n ≥ 3 neurons.

Mol. Cells 2020;43:870~879 https://doi.org/10.14348/molcells.2020.0158

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