Molecules and Cells

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Fig. 3.

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Fig. 3. Deletion of Wdr1 inhibits neointima formation. (A and B) Western blot and averaged data showing the levels of WDR1 after 5 days of tamoxifen treatment, with male littermate Wdr1 f/+ mice as controls. (C and D) Representative photomicrographs of H&E staining and averaged data of neointima/media ratio in carotid arteries from Wdr1 f/+;ERT2Cre and Wdr1 f/+ control mice 14 days after CAL. N, neointima; M, media. Scale bars = 20 μm. (E and F) Immunofluorescence staining of α-SMA and PH3. The yellow dotted lines represent the boundary between the arterial tunica media and the intima. Scale bar = 50 μm. Statistical results show that the number of PH3-positive cells is significantly less in Wdr1 f/+;ERT2Cre mice than Wdr1 f/+ animals. (G and H) The level of MMP9 is significantly less in Wdr1 f/+;ERT2Cre mice after left common CAL compared with that in Wdr1 f/+ animals. Data are expressed as mean ± SEM (n = 4 per group). *P < 0.05; **P < 0.01.
Mol. Cells 2020;43:749~762
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