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Fig. 6.

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Fig. 6. GBM-induced polarization of microglia/macrophages. (A) Immunostaining with anti-Iba1 showing the higher level of microglia/macrophages in the peri-tumoral area (a boundary between zones 2 and 3) with U87/GFP cells compared to the LN229/GFP cells. Note the difference for the IL-1β (a M1 type marker), and CD206 (a M2 type marker) in the peri-tumoral region. Scale bar = 100 µm. (B and C) Quantiļ¬cation of the number of cells that were immunoreactive to antibodies specific for Iba1 (B) and IL-1β and CD206 (C). The data are presented as mean ± SE per mm2 obtained from three animals per group. **P < 0.01. (D and E) RT-PCR analysis showing differential gene expression of NOS2 and ARG1 in BV2 cells following co-culture with U87/GFP or LN229/GFP cells. The data present mean ± SE from three independent experiments. *P < 0.05, **P < 0.01.
Mol. Cells 2020;43:539~550
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