Molecules and Cells

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Fig. 4.

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Fig. 4. In vitro migration and invasion assays. (A) Fluorescence microscopic images of wound closure of U87/GFP and LN229/GFP cells cultured to a monolayer at the indicated time after scratching with a pipette. Scale bar = 400 µm. (B) Quantification of GFP intensity in the scratched area at the indicated time. Results from three independent experiments are presented as mean ± SE. *P < 0.05 compared to LN229/GFP at indicated time. (C) Schematic illustration of the transwell invasion assays. Representative fluorescence images of U87/GFP and LN229/GFP cells with (w/) or without (w/o) Matrigel in the upper chamber and bEND.3 cells or CM in the lower chamber. (D and E) The intensity of GFP levels measured at the bottom surface of membrane of the upper chamber. The data are presented as mean ± SE from three independent experiments. *P < 0.05, **P < 0.01.
Mol. Cells 2020;43:539~550
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