Molecules and Cells

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Fig. 3.

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Fig. 3. C3H10T1/2 cells were transfected with siFTO (+, 100 nM; ++, 200 nM) for 6 h and then treated with BMP2 (0.25 μg/ml) for 1 day. (A) RT-PCR analysis was performed using total RNA isolated from cells. (B) The real-time PCR analysis was performed using total RNA isolated from cells. (C) Western blot analysis was performed using the indicated antibodies. (D) C3H10T1/2 cells were transfected with siFTO for 6 h and then treated with BMP2 (0.25 μg/ml) for 4 days. ALP staining was performed. All experiments were independently repeated at least three times. Data represent the mean ± SEM of three individual experiments. *P < 0.05 and **P < 0.001 compared with untreated control cells. #P < 0.05, ##P < 0.01, and ###P < 0.005 compared with BMP2 treatment cells. All experiments were independently repeated at least three times.
Mol. Cells 2020;43:58~65 https://doi.org/10.14348/molcells.2019.0136
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