Molecules and Cells

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Fig. 7.

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Fig. 7. (A) Schematic diagrams of OsTB1 (left) and OsVIL2 (right) genes. The positions of mutation target sites are indicated with arrows. (B) Schematic diagram of the polycistronic tRNA-gRNA (PTG)/Cas9 vector for targeting multiple sites. The synthetic PTG consists of tandemly arrayed tRNA-gRNA units. (C) Mutated sites in OsTB1. Target sequences are indicated in red. Deleted sequences are indicated by =. (D) Mutated sites in OsVIL2. Target sequences are indicated in red. Deleted sequences are indicated by =. (E) Tiller phenotypes of osvil2-1, ostb1, and osvil2 ostb1 double mutants. As a control, we generated transgenic plants with pRGEB32 empty vector. Phenotypes were observed immediately before heading stages. Scale bars = 10 cm. (F) Number of tiller in control, osvil2-1, ostb1, and osvil2 ostb1 double mutant. Error bars are SD (n = 6).
Mol. Cells 2019;42:858~868 https://doi.org/10.14348/molcells.2019.0141
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