Molecules and Cells

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Fig. 2.

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Fig. 2. (A–D) BMMs were transfected with control siRNA or ST5 siRNA, and cells were stimulated with a medium containing M-CSF (30 ng/ml) and RANKL (150 ng/ml). (A) When MNCs were formed at day 4, cells were stained for TRAP activity. The images were captured by a light microscope, and TRAP-positive MNCs (≥ 3 nuclei) were counted as osteoclasts. Scale bars = 200 μm. (B) To examine the resorptive activity of osteoclasts, transfected BMMs were treated with M-CSF and RANKL on dentine discs for 7 to 9 days. Dentine discs were analyzed with a confocal microscope. Representative images of dentine surfaces (left), and values of resorbed depth and area of resorptive pits are presented (right). (C) The mRNA expression levels of St5, c-Fos, Nfatc1, and Acp5 were analyzed by real-time PCR. *P < 0.05, **P < 0.01, ***P < 0.001 (by t-test). (D) The levels of c-Fos and NFATc1 expression were detected by Western blotting.
Mol. Cells 2019;42:810~819
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