Molecules and Cells

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Fig. 2.

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Fig. 2. (A–D) Knockdown of HOTTIP partially rescues the level of G2/M phase arrest (A and B) and early apoptosis (C and D) in UV-stimulated GC-1 cells. GC-1 cells were transfected with si-NC or si-HOTTIP, and were treated using 0, 5, and 10 J/m2 UV 24-h post transfection. 24 h subsequently, cell cycle (A and B) and apoptosis (C and D) of GC-1 cells were detected by using FACS. Representative photographs of cell cycle and apoptosis were shown in panels (B) and (D), respectively. (E) HOTTIP inhibited the expression of proteins that regulate UV-induced cell cycle arrest, apoptosis and DNA damage repair in GC-1 cells. GC-1 cells were transfected with si-NC or si-HOTTIP, and were treated using 0, 5, and 10 J/m2 UV 24-h post transfection. Protein abundance was analyzed using western blotting. (A and C) Data shown are the mean ± SEM of three independent experiments each performed in triplicate. *P < 0.05, **P < 0.01.
Mol. Cells 2019;42:794~803 https://doi.org/10.14348/molcells.2019.0121
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