Molecules and Cells

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Fig. 1.

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Fig. 1. (A and B) The coordinated expression of lncRNA-HOTTIP and Hoxa13. RT-qPCR was used to examine expression of both HOTTIP and Hoxa13 in mouse tissues (A) and cell lines (B). (C) LncRNA-HOTTIP promotes the expression of Hoxa13. The efficiency of the knockdown of HOTTIP and the Hoxa13 expression was confirmed using RT-qPCR to measure transcript levels in GC-1 cells after they had been transfected with si-NC or si-HOTTIP for 24 h. (D) UV induces the coordinated expression of lncRNA-HOTTIP and Hoxa13. GC-1 cells were treated with 0, 5, and 10 J/m2 UV. Expression levels of genes were then measured using RT-qPCR 24-h post treatment. (E) Knockdown of HOTTIP attenuates the inhibitory effects of UV treatment on GC-1 cell proliferation. GC-1 cells were transfected with si-NC or si-HOTTIP and were treated with 0, 5, and 10 J/m2 UV 24-h post transfection. Proliferation of GC-1 cells was measured by using the CCK-8 method 24-h post UV exposure. Data shown are the mean ± SEM of three independent experiments, each of which were performed in triplicate. *P < 0.05.
Mol. Cells 2019;42:794~803 https://doi.org/10.14348/molcells.2019.0121
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