Molecules and Cells

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Fig. 4.

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Fig. 4. (A) HLR-GV-hATF6α(333) cells were stained with Hoechst 33342 (blue fluorescence) and anti-Gal4 antibody (red fluorescence) after DTT treatment for 6 h and analyzed by confocal fluorescence microscopy. Scale bars = 30 μm. (B) HLR-GV-hATF6α(333) cells were treated with DTT (2 mM), Tm (1 μg/ml), or Tg (5 nM) for 12 h. Cytosolic and nuclear fractions extracted from HLR-GV-hATF6α(333) cells treated as indicated were subjected to Western blot analysis using anti-Gal4, anti-α-tubulin, and anti-histone H3 antibodies.
Mol. Cells 2019;42:783~793
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