Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Graphical representation of the pCMV-GV-hATF6αΔN(aa 333–670) plasmid described in Materials and Methods section. (B) Graphical representation of a double-stable HLR cell line having both pGAL4USA-Luc plasmid and pCMV-GV-hATF6αΔN(aa 333–670) plasmid stably integrated into the genome. (C) Both hygromycin B (0.1 mg/ml) and G418 (0.5 mg/ml)-resistant stable cell clones were isolated. These clones were treated with DTT (2 mM) as described in Materials and Methods section. Firefly luciferase activities in cell lysates were measured. Results are given as absolute values of firefly luciferase activity to 1 μg of proteins in each cell lysate. The number in parenthesis indicates average fold induction relative to the untreated group. Data are expressed as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 (untreated group vs each treated group); n.s., not significant.
Mol. Cells 2019;42:783~793
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