Molecules and Cells

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Fig. 1.

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Fig. 1. (A) Isolation and culture of primary periodontal ligament cells (PDLCs). Spindle-shaped adherent cells appeared from the primary explants. Scale bar = 100 μm. Confluence of 80% was reached before passage. Scale bar = 500 μm. Putative stem cells were identified by a single-cell colony formation assay and stained with crystal violet. Scale bar = 500 μm. (B) PDLCs showed multilineage differentiation potential. Compared to that of the control group, Alizarin Red S staining revealed several red calcified nodules, and Oil Red O staining showed several red fat droplets inside PDLCs, aside from the changed cell shapes. All scale bars = 100 μm. (C) Flow cytometry showed that the PDLCs expressed the MSC surface markers CD44 and CD29 and were negative for the haematopoietic cell surface markers CD34 and CD45.
Mol. Cells 2019;42:763~772 https://doi.org/10.14348/molcells.2019.0023
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