Fig. 1. (A) LR lysates were fractionated using a three-step chromatography approach. (B) To determine the anti-cancer activity of each fraction, each was tested in a cell proliferation assay based on DLD-1 and HT-29 cells. (C) The anti-cancer properties of fraction A2 were confirmed by fluorescence microscopy at ×200 magnification. Green, live cells; Red, dead cells. Scale bars = 200 μm. (D) Each fraction was separated by SDS-PAGE and stained with Coomassie blue-250. P8 protein of each fraction was determined by western blotting.

Mol. Cells 2019;42:755~762 https://doi.org/10.14348/molcells.2019.0064

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