Molecules and Cells

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Fig. 1.

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Fig. 1. CellTrace-Violet (CTV)-labelled human PBMCs were activated with 3 μg/ml soluble α-CD3 and 3 μg/ml soluble α-CD28 antibody in culture media alone or supplemented with the indicated doses of DMF for four days. (A) Representative flow cytometric plot of proliferating (CTVlow) cells that is gated on live CD4+ and CD8+ T cells. (B) Frequency of proliferating (CTVlow) CD4+ and CD8+ T cells as in A. (C) CTV profile showing proliferation versus IFN-γ gating on live CD4+ and CD8+ T cells. (D) Frequency of IFN-γ-secreting cells among live CD4+ and CD8+ T cells, as in C. Results are representative of three independent experiments. Data are shown as mean ± SEM and statistical significance was determined using a two-tailed unpaired Student’s t-test; *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.
Mol. Cells 2019;42:597~603
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