Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Expression of βPix protein in WT, βPix-KO MEFs (KO1 and KO2 without Cre), and βPix-rescue MEFs (KO1 and KO2 with Cre). Equal amounts of 12 μg lysates were loaded per lane. (B) Active Rac1 level determined by GST-PBD pull-down assay. PD, pull-down; TCL, total cell lysate. (C and D) Representative images for the MEFs transfected with GFP (C) and quantification of cell spreading area (D). At least 100 cells from 4 independent experiments were analyzed in each group. (E) Ruffle formation in response to PDGF in the MEFs. Cells (2698–5697) from 4 independent experiments were analyzed in each group. (F) Chemotaxis in response to PDGF. The number of migratory cells was counted in 134–268 random fields from 7 independent experiments in each group. *P < 0.05, **P < 0.01, ***P < 0.001.
Mol. Cells 2019;42:589~596 https://doi.org/10.14348/molcells.2019.0140
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