Molecules and Cells

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Fig. 3.

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Fig. 3. (A) Radiation induces c/EBPβ recruitment to the CXCL12 promoter in both single- or cocultured Huh7 cells. ChIP assays were carried out using anti-c/EBPβ antibody 1 to 5 days after treating Huh7 cells with or without γ-irradiation. The pulled-down chromatin pools were PCR amplified with primers targeting the CXCL12 promoter. The pulled-down chromatin samples immediately before radiation (0 day) were arbitrarily set to one. (B) The c/EBPβ knockdown levels were confirmed using western blotting. (C) The c/EBPβ knockdown alleviated radiation-induced CXCL12 expression. Time course analysis of CXCL12 mRNA levels were performed following radiation in Huh7 cells transfected with an empty (mock) or c/EBPβ silencing (sic/EBPβ) vectors one day earlier. The CXCL12 mRNA levels in non-transfected and non-irradiated Huh7 cells were arbitrarily set to one. *P < 0.05, **P < 0.01, ***P < 0.001.
Mol. Cells 2019;42:530~545
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