Molecules and Cells

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Fig. 4.

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Fig. 4. (A) Expression changes of miR-142-5p in H9c2 cells were detected by RT-qPCR, after transfection with miR-142-5p inhibitor or its negative control (NC). H9c2 cells were transfected with sh-GAS5 alone or in combination with miR-142-5p inhibitor. Then, the cells were exposed to hypoxic condition for 6 h. (B) Cell viability, (C–D) protein expression changes of p53, CyclinD1, and CDK4, (E) apoptosis rate, and (F–G) protein expression changes of apoptosis-related factors were assessed by CCK-8 assay, flow cytometer and Western blot. Cells without any treatment were used as control. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the indicated group.
Mol. Cells 2019;42:397~405
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