Molecules and Cells

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Fig. 3.

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Fig. 3. (A) Expression changes of heart failure-related miRNAs (miR-378, miR-21, miR-30b, miR-93, and miR-142-5p) in H9c2 cells were detected by RT-qPCR, after transfection with sh-NC or sh-GAS5. Untransfected cells served as control. (B) Schematic representation of the miR-142-5p site in lncRNA GAS5. (C) A construct containing lncRNA GAS5 transcripts combined with MS2 elements. (D) MS2-RIP followed by qPCR to assay miR-142-5p endogenously associated with lncRNA GAS5. (E) AGO-RIP followed by qPCR to assay lncRNA GAS5 endogenously associated with miR-142-5p. (F) H9c2 cells transfected with sh-GAS5 or sh-NC were treated by hypoxia, and then the expression changes of miR-142-5p were measured by RT-qPCR. Cells without any treatment were used as control. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the indicated group.
Mol. Cells 2019;42:397~405
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