Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Expression changes of lncRNA GAS5 in H9c2 cells were detected by RT-qPCR, after transfection with sh-NC or sh-GAS5. Untransfected cells served as control. Then, the transfected cells were exposed to hypoxia for 6 h, after which (B) viability, (C–D) protein expression changes of p53, CyclinD1, and CDK4, (E) apoptosis rate, and (F–G) protein expression changes of apoptosis-related factors were assessed by CCK-8 assay, flow cytometer and Western blot. Cells without any treatment were used as control. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the indicated group.
Mol. Cells 2019;42:397~405
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