Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Relative PVT1 expression was detected by qRT-PCR in cultured mouse primary VSMCs that were stimulated with different concentrations of Ang II (0, 1, 5, and 10 μM) at various time points (0, 12, 24, and 48 h). *P < 0.05, **P < 0.01 vs. 0 h group. The cultured VSMCs were then induced with Ang II (10 μM, 48 h) alone or transfected with the indicated vectors. (B) The apoptosis rate of cultured VSMCs was examined by flow cytometry. A representative flow cytometry plot of cultured VSMCs from each group is shown. **P < 0.01 vs. control group, ##P < 0.01 vs. Ang II+OE-Ctrl group, $$P < 0.01 vs. Ang II+sh-Ctrl group. Ang II: Angiotensin II; OE-Ctrl: overexpression control; PVT1: PVT1 overexpression.
Mol. Cells 2019;42:218~227
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