Molecules and Cells

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Fig. 4.

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Fig. 4. CellTrace Violet (CTV)-labeled small intestinal lamina propria (sLP) CD4 T cells from either GF- or SFB-monocolonized B6 mice were cultured. (A) Histograms show CTV-dilution profile of co-cultured CD4 T cells in the presence of GF fecal antigen, GF fecal Ag plus anti-CD3 antibody (2 μg/ml), or SFB fecal antigen. Dot plots show CTV-dilution and SSC pattern. (B) Dot plots showing RORγt and Foxp3 expression in sLP CD4 T cells from GF B6 mice (top) or GF B6 mice monocolonized with SFB 2 weeks before (bottom) (C and D). Dot plots showing phenotype of co-cultured T cells after proliferation in the presence of GF fecal Ag plus anti-CD3 antibody (2 μg/ml) or SFB fecal antigen alone. (C) CTV dilution profile and RORγt expression in co-cultured T cells. (D) Foxp3 and RORγt expression in CTVneg SFB-specific T cells (left). IFNγ and IL-17A expression in CTVneg SFB-specific T cells after 4 h re-stimulation with PMA/ionomycin in the presence of protein transport inhibitors (right). Numbers indicate frequency of cells in adjacent gates or in each quadrant. Representative data from at least two independent experiments are shown.
Mol. Cells 2019;42:228~236 https://doi.org/10.14348/molcells.2018.0424
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