Molecules and Cells

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Fig. 1.

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Fig. 1. Methylation status and expression of the TGFBI gene were analysed via MSP and RT-PCR analyses, respectively. (A) Tumour tissues and matched non-malignant lung tissue specimens showed methylated (205N, 205T, and 246T) or unmethylated alleles (200N, 200T, 205N, 205T, 246N, 246T, 256N, and 256T). CpGenomeTM Universal methylated and unmethylated DNA was used as positive control for the methylated and unmethylated products, respectively. Distilled water was used as negative control. N, non-malignant tissue; T, tumor tissues; U and M, amplified products using primers targeting unmethylated or methylated sequences. TGFBI mRNAs were markedly decreased in 205N, 205T, and 246T tissues with methylated promoter. GAPDH was used as an internal loading control. (B) (−) indicated vehicle alone treatment and (+) indicated 20 μM 5-aza-2′-deoxycytidine (5-AzadC) treatment for 3 days.
Mol. Cells 2019;42:161~165 https://doi.org/10.14348/molcells.2018.0322
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