Molecules and Cells

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Fig. 7.

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Fig. 7. (A) Interaction of USP44 and gain-of-function EZH2 mutants was verified by immunoprecipitation. (B) HEK293T cells were transfected with Flag-USP44 and HA-tagged gain-of-function EZH2 mutants. Equal amounts of protein from each cell lysate were immunoblotted to detect HA-EZH2 mutant and Flag-USP44. (C) HEK293T cells were transfected with shUSP44 and HA-tagged gain-of-function EZH2 mutants. Equal amounts of protein from each cell lysate were immunoblotted to detect HA-EZH2 mutant and USP44. (D) Interaction of USP44 and therapy-resistant EZH2 mutants was verified by immunoprecipitation. (E) HEK293T cells were transfected with Flag-USP44 and therapy-resistant HA-EZH2 mutants. Equal amounts of protein from each cell lysate were immunoblotted to detect HA-EZH2 mutant and Flag-USP44. (F) HEK293T cells were transfected with shUSP44 and HA-tagged therapy-resistant EZH2 mutants. Equal amounts of protein from each cell lysate were immunoblotted to detect HA-EZH2 mutant and USP44.
Mol. Cells 2019;42:17~27 https://doi.org/10.14348/molcells.2018.0329
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