Molecules and Cells

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Fig. 5.

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Fig. 5. (A) Expression of pluripotent CSC markers including Oct4, Nanog, Bmi1, Klf4, and Sox2 were examined by immunoblotting in USP44 knockdown stable cell lines. (B) Real-time RT-PCR analysis on the transcript levels of CD44 and CD133 in USP44 knockdown stable cell lines. Values are expressed as the mean ± SD of three independent experiments. (C) Self-renewal potency was evaluated by the formation of tumor spheres in USP44 knockdown stable cell lines. Stable cells were seeded in 24-well Ultra-low Attachment plates and cultured in serum-free media. The number of spheres larger than 200 μm in diameter was counted. The figure shows the representative images of each stable cell line and each scale bar is 200 μm. Values are expressed as the mean ± SD of three independent experiments. (D) Equal numbers of cells from USP44 knockdown stable cell lines were seeded into 6-well plates. Twenty-four hours after seeding, the cells were treated with varying concentrations of doxorubicin or etoposide. Twenty-four hours after treatment, viable cells were counted by the trypan blue-exclusion assay. The p value is shown from a Student’s t-test. *p < 0.05, **p < 0.01
Mol. Cells 2019;42:17~27 https://doi.org/10.14348/molcells.2018.0329
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