Molecules and Cells

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Fig. 2.

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Fig. 2. (A) HEK293T cells were transfected as indicated and each cell lysate was immunoblotted to detect HA-EZH2 and Flag-USP44. (B) USP44 shRNA was transfected as indicated and protein from each cell lysate was immunoblotted to detect EZH2 and USP44. Line graph representing the relative protein levels of EZH2 normalized to β-actin. (C) Mock or Flag-USP44/USP44 C282A-expressing HEK293T cells were treated with cycloheximide (CHX) for the indicated times. Equal amounts of protein were used to detect EZH2 by immunoblotting. Line graph representing the relative protein levels of EZH2 normalized to β-actin using a densitometer and expressed as the relative intensity compared to the non-treated control. (D) HEK293T cells were transfected as indicated and treated with MG132 for 12 h before harvesting. Each cell lysate was subjected to Ni-NTA pulldown followed by immunoblotting with HA antibody. (E) HEK293T cells were transfected as indicated and treated with MG132 for 12 h before harvesting. Each cell lysate was subjected to immunoprecipitation with HA antibody followed by immunoblotting with Xpress and HA antibodies.
Mol. Cells 2019;42:17~27 https://doi.org/10.14348/molcells.2018.0329
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