Molecules and Cells

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Fig. 5.

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Fig. 5. (A–F) Splenic lymphocytes were isolated by grinding and placed in circulating medium with or without 10 μg/ml Con A, followed by in situ liver perfusion for 1 h. (A) Schematic figure of the experiment. (B) Expression of Ifng and Il6 mRNAs by isolated splenic lymphocytes. (C) Immunoblot assays for pSTAT1 and STAT1 in whole liver tissues after perfusion. Full-length blots are presented in . (D) Expression of Ccl2 and Tnf mRNAs by whole liver tissues after perfusion. (E) ALT levels in circulating medium. (F) Representative TUNEL staining after perfusion. Scale bars = 50 μm. (G) Numbers of apoptotic cells assessed by TUNEL staining. Gene expression was normalized relative to that of Actb. Data are shown as mean ± sem. *P < 0.05, **P < 0.01, ***P < 0.001 versus the corresponding control.
Mol. Cells 2019;42:45~55
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