Fig. 4. (A) Schematic figure of the experiments. (B–C) In situ liver perfusion with medium with or without IFN-γ (5 ng/ml) for 1 h. (B) Immunoblot assays for pSTAT1 and STAT1. Full-length blots are presented in . (C) Expression of Irf1, Socs1, and Nos2 mRNAs in whole liver tissues. (D–E) In situ liver perfusion with medium with or without poly I:C (0.5 μg/ml) for 1 h. (D) Expression of Il1b, Il6, Il17a, and Ifng mRNAs by isolated hepatic immune cells. (E) Flow cytometric analyses of isolated hepatic immune cells. Gene expression was normalized relative to that of Actb. Data are shown as mean ± sem. *P < 0.05, **P < 0.01 versus the corresponding control.

Mol. Cells 2019;42:45~55 https://doi.org/10.14348/molcells.2018.0330

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