Molecules and Cells

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Fig. 3.

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Fig. 3. (A–C) Mice were sacrificed and perfused in situ with DMEM containing 10% FBS and 1% penicillin-streptomycin or not perfused. (A) Representative H&E and TUNEL staining. Scale bars = 50 μm. (B) Numbers of apoptotic cells assessed by TUNEL staining. (C) Levels of hepatic Alb, Cyp2e1, and G6pc mRNAs. (D–G) Mouse livers were perfused in situ with medium with (EtOH) or without (Ctrl) 100 mM ethanol for 2 h. (D) ALT levels in the medium. (E) Immunoblot assays for CYP2E1. Full-length blots are presented in . (F) qRT-PCR assays for Cyp2e1, Adh1, and Adh5. (G) Representative CYP2E1 immunohistochemical staining. Scale bars = 50 μm. Gene expression was normalized relative to that of Actb. Data are shown as mean ± sem. *P < 0.05, **P < 0.01, ***P < 0.001 versus the corresponding control.
Mol. Cells 2019;42:45~55
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