Molecules and Cells

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 0219-1032   pISSN 1016-8478

Fig. 2.

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Fig. 2. (A) Immunolabeling results to identify osteogenic related proteins contrasting between the hMSCs beads, BMP-2 beads, guest BMP-2 beads and BM beads. After two weeks culture, collagen I expression was stronger in the BMP-2 added group and BM added group compared to control group and guest BMP-2 group. The CD 90 expression was absent in all groups after two weeks culture. Thus, all encapsulating hMSCs lost stemness after two weeks culture. MSCs expressed Osterix, the marker for pre-osteoblasts, in alginate, and at higher levels in the BMP-2 and BM beads. Runx-2 staining was positive in the BMP-2 added, guest BMP-2 added and the BM beads, and absent in the control group. The osteocalcin cell expression in the BM beads compared with alginate beads, BMP-2 beads and nested BMP-2 beads; (scale bar = 400 μm); (B) percentages of positively versus negatively stained cells for Runx-2, osteocalcin, and CD90 to show the maturation phase of the hMSCs and their conversion into osteogenic lineage cells. (* = Guest BMP-2 bead). (C) Masson’s trichrome staining shows the in vivo generated structures from the four different kinds of alginate bead transplants within a mouse cranial bone defect. We find a difference between the MSC control beads inside the defect, and the added BMP-2 and BM alginate beads, which both show a narrowing of the pockets and contain osteoid tissue. More significantly, we see that the beads harboring a BMP-2 guest bead generated an extensive series of osteoid pockets across the defect region (C) accompanied by an even tighter narrowing of the regenerated tissue inside the defect. The IHC staining for OCN and OPN is negative among the control MSC beads. In contrast to the MSC beads, BMP-2 and BM laden beads were OPN and OCN positive. The spaces represent the alginate. Among the defects containing nested alginate beads, the staining for OCN and OPN was larger, more widespread and associated with the osteoid pockets (C; * = Guest BMP-2 bead; black arrows: host bone, yellow areas: newly formed hard tissue) (Scale bar = 200 μm).
Mol. Cells 2018;41:1016~1023 https://doi.org/10.14348/molcells.2018.1001
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