Molecules and Cells

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Fig. 2.

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Fig. 2. (A) NCI-H157 cells were treated with PS-341 (50 nM) or MG132 (20 μM) for the indicated times. Total cellular extracts were subjected to Western blot analysis for Lamp2a, Lamp2, LC3B, and GAPDH. (B) Cells were stimulated with PS-341 (50 nM) for the indicated times. Total RNA was isolated and quantitative real-time PCR for LC3B and GAPDH was performed. Data represent the mean ± SD of triplicate experiments. **P < 0.05 (C) NCI-H157 cells were pretreated with 3-methyladenine (3-MA, 5 and 10 mM) for 1 h and then stimulated with PS-341 or MG132 in the presence or absence of 3-MA for 6 h. Total cellular extracts were subjected to Western blot analysis for IκBα and GAPDH. Results are representative of three independent experiments.
Mol. Cells 2018;41:1008~1015 https://doi.org/10.14348/molcells.2018.0277
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