Molecules and Cells

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Fig. 1.

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Fig. 1. (A) NCI-H157 and A549 cells were treated with PS-341 (50 nM) or MG132 (20 μM) for the indicated times. Total cellular extracts were subjected to Western blot analysis for IκBα and GAPDH. (B) NCI-H157 cells were treated with PS-341 or MG132 for the indicated times. Nuclear proteins were extracted and subjected to Western blot analysis for p65 and Lamin A/C. TNF-α (10 ng/mL, 60 min) was used as a positive control. (C) Both cell lines were stimulated with PS-341 or MG132 for 0, 6, 8, 12, or 24 h. Total RNA was isolated and quantitative real-time PCR for COX-2 and GAPDH was performed. Data represent the mean ± SD of triplicate experiments. **P < 0.05 (D) NCI-H157 cells were treated with PS-341 or MG132 for the indicated times. Total cellular extracts were subjected to Western blot analysis for COX-2, cIAP2, and GAPDH. (E) NCI-H157 cells were pretreated with DHMEQ (10 or 20 μg/mL) for 2 h and then stimulated with PS-341 for 6 or 24 h. Nuclear proteins (6 h) and whole cell proteins (24 h) were isolated and subjected to Western blot analysis for p65, Lamin A/C, COX-2, and GAPDH. (F) NCI-H157 cells were transiently transfected with control siRNAs or p65 siRNAs. Forty-eight hours after transfection, the cells were stimulated with PS-341 for 24 h. Total cellular extracts were subjected to Western blot analysis for COX-2, p65, and GAPDH. Results are representative of three distinct experiments.
Mol. Cells 2018;41:1008~1015 https://doi.org/10.14348/molcells.2018.0277
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