Molecules and Cells

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Fig. 1.

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Fig. 1. (A) Genomic organization of the mkrn1 locus. The insertion site of P-element P{EY14602} is indicated by a white triangle. P1 and P2 denote primers used for genomic DNA PCR analysis in (B). Exons and introns are indicated by black and white rectangles, respectively. By mobilizing the P element, the two deletion alleles mkrn1exS and mkrn1exL were isolated. (B) Deletion was confirmed by genomic DNA PCR using primers P1 and P2. A precise excision line (CTRL) was isolated and used as a control. (C) RT-PCR analysis was performed with CTRL and mkrn1exS alleles. In the mkrn1exS allele, mkrn1 was not expressed, whereas the expression of nearby genes CG32442 and ppk5 was similar to those in control flies.
Mol. Cells 2018;41:1024~1032 https://doi.org/10.14348/molcells.2018.0367
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