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Fig. 4.

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Fig. 4. (A) HUVECs were exposed to L-flow (12 dynes/cm2) for 24 h, then incubated with 300 μM L-NAME for 1 h before treatment with 1 μM TG for 3 h. Protein level was analyzed by immunoblotting with specific antibodies against PARP-1 Cleaved Caspa-se-3 and α-Tubulin. Bar graphs present the densitometric quantifications of Western blot bands. ANOVA: *p < 0.05; **p < 0.01. (B) Protein level was analyzed by immunoblotting with specific antibodies against spliced-XBP1, ATF4, CHOP, p-eIF2α and α-Tubulin. The asterisk (*) refers to a non-specific band detected by the anti-ATF4 antibody. Bar graphs present the densitometric quantifications of Western blot bands. ANOVA: *p < 0.05; **p < 0.01; N.S (Not significant) (n = 3).
Mol. Cells 2018;41:964~970 https://doi.org/10.14348/molcells.2018.0111
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