Molecules and Cells

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Fig. 4.

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Fig. 4. (A, B) Levels of PTBP1 mRNA (A) and the protein (B) in βIRWT and βIRKO cells. All mRNA amounts were normalized to the levels of 18S rRNA. Relative densities for the indicated proteins are shown at the right. *P < 0.05, **P < 0.01 versus the βIRWT group using Student’s t-test. (C) Levels of PTBP1 and IRβ in βTC6 cells transiently transfected with the indicated siRNAs. Relative densities for the indicated proteins are shown at the right. **P < 0.01 versus the Ctrl siRNA group using Student’s t-test. (D) Diagram of constructs for Flag-tagged IR-WT and IR-3YA, in which the three autophosphorylation sites (Tyr1158/1162/1163) are substituted with Ala. (E) Western blot analysis for the indicated proteins in βIRKO cells transfected with empty vector, IR-WT, or IR-3YA. Relative densities for the indicated proteins are shown at the right. **P < 0.01 versus the vector group using Student’s t-test. (F) Levels of PTBP1 and Akt in βTC6 cells transfected with the indicated siRNAs. Relative densities for the indicated proteins are shown at the right. **P < 0.01 versus the Ctrl siRNA group using Student’s t-test. All data represent the mean ± SEM from three independent experiments. (G) Schematic illustration of the proposed mechanism by which IR signaling pathway regulates PTBP1 expression in pancreatic β cells.
Mol. Cells 2018;41:909~916 https://doi.org/10.14348/molcells.2018.0147
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