Molecules and Cells

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Fig. 2.

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Fig. 2. (A, B) Levels of PTBP1 mRNA (A) and the protein (B) in βTC6 cells treated with glucose. βTC6 cells were cultured for 12 h in 5 mM glucose + 0.1% FBS and further cultured for 12 h with the indicated concentrations of glucose. Levels of PTBP1 mRNA and the protein were assessed by RT-qPCR analysis and western blot analysis, respectively. All mRNA amounts were normalized to the levels of 18S rRNA. (C, D) Levels of PTBP1 mRNA (C) and the protein (D) in βTC6 cells treated with insulin. βTC6 cells were cultured for 12 h in 5 mM glucose + 0.1% FBS and further cultured for 12 h with the indicated concentrations of insulin. Levels of PTBP1 mRNA and the protein were assessed by RT-qPCR analysis and western blot analysis, respectively. All mRNA amounts were normalized to the levels of 18S rRNA. Data represent the mean ± SEM from at least three independent experiments. *P < 0.05, **P < 0.01 versus the 5 mM group (A, B) or the vehicle group (C, D) using Student’s t-test.
Mol. Cells 2018;41:909~916 https://doi.org/10.14348/molcells.2018.0147
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