Molecules and Cells

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Fig. 1.

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Fig. 1. A) Representative images of immunostaining for PTBP1 (red), insulin (green), and DAPI (blue) in mouse pancreatic islets. Boxed areas are magnified and shown in the bottom panel. Scale bars, 50 μm. (B) Representative images of DAB staining for PTBP1 in monkey pancreatic islets. Boxed areas are magnified and shown in the bottom panel. Scale bars, 50 μm. (C) Levels of proinsulin and β-actin loading control in βIRWT cells transiently transfected with the indicated siRNAs. Relative densities for the indicated proteins are shown at the right. **P < 0.01 versus Ctrl siRNA group using Student’s t-test. (D) Effects of PTBP1 knockdown on glucose-stimulated insulin secretion in βTC6 cells. *P < 0.05, **P < 0.01 versus the 5 mM of Ctrl siRNA group; #P < 0.05, ##P < 0.01 versus the 5 mM of PTBP1 siRNA group using Student’s t-test. (E) Levels of proinsulin and β-actin in βTC6 cells transiently transfected with either empty vector (Vector) or PTBP1 cDNA construct (PTBP1). Relative densities for the indicated proteins are shown at the right. *P < 0.01 versus Vector group using Student’s t-test. (F) Effects of PTBP1 overexpression on glucose-stimulated insulin secretion in βTC6 cells. *P < 0.05, **P < 0.01 versus the 5 mM of Vector group; #P < 0.05, ##P < 0.01 versus the 5 mM of PTBP1 group using Student’s t-test. All data represent the mean ± SEM from at least three independent experiments.
Mol. Cells 2018;41:909~916 https://doi.org/10.14348/molcells.2018.0147
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