Molecules and Cells

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Fig. 3.

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Fig. 3. (A) Schematic of optoSTIM1activation by blue light-inducible PHR homo-oligomerization. A red-shifted calcium reporter, R-GECO can be simultaneously visualized under the precise control of spatiotemporal OptoSTIM1 activation. (B) Pseudocolored fluorescence images of a red-shifted genetically encoded calcium indicator (R-GECO1). Three HeLa cells (R1, R2, and R3) co-expressing OptoSTIM1 and R-GECO1 were sequentially stimulated with blue light at different time points. White dotted lines indicate cell boundaries. Scale bar, 20 μm. (C) Pseudocolor image of R-GECO1PM fluorescence in a HeLa cell co-expressing OptoSTIM1. White circles designate three subcellular regions (R1, R2, and R3) sequentially stimulated with blue light. Scale bar, 20 μm. Graph represents changes in fold-changes of R-GECO1PM according to time at three subcellular regions. Red, green and blue arrows indicate time points of light stimulation in R1, R2 and R3, respectively.
Mol. Cells 2018;41:809~817
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