Molecules and Cells

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Fig. 3.

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Fig. 3. The KLF4 mRNA and protein expression were examined by qRT-PCR (A, C, and I) and western blotting (B, D, and H). ALP activity was detected by a commercial Alkaline Phosphatase Detection Kit (E, J). The genes expression of osteogenic marker including Collagen I, Runx2 and Osterix were examined by Western blotting (F, K) and qRT-PCR (G, L). pcSOX5+siKLF4: cells were transfected with recombinant plasmid pcDNA3.1/SOX5 (pcSOX5) and KLF4 siRNA, and then cultured with OM. TNF-α+siKLF4: cells were transfected with KLF4 siRNA and then cultured with OM containing TNF-α. Data are expressed as the mean ± SD, n = 3. *P < 0.05.
Mol. Cells 2018;41:575~581
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