Molecules and Cells

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Fig. 2.

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Fig. 2. The SOX5 mRNA and protein expression were examined by qRT-PCR (A) and western blotting (B). ALP activity was detected by a commercial Alkaline Phosphatase Detection Kit (C). The genes expression of osteogenic marker including Collagen I, Runx2 and Osterix were examined by Western blotting (D) and qRTPCR (E). TNF-α: cells were cultured with OM containing TNF-α; TNF-α+siSOX5: cells were transfected with SOX5 siRNA and then cultured with OM containing TNF-α. Data are expressed as the mean ± SD, n = 3. *P < 0.05.
Mol. Cells 2018;41:575~581 https://doi.org/10.14348/molcells.2018.2359
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