Molecules and Cells

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Fig. 1.

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Fig. 1. SOX5 mRNA and protein expression were examined by qRTPCR (A) and western blotting (B) in cells cultured with OM for 0 days (control) and 14 days (OM). Cells were transfected with recombinant plasmid pcDNA3.1/SOX5 (pcSOX5) and its negative control plasmid (pcNC), or SOX5 siRNA (siSOX5) and its negative control (siNC) for 15 h, after which the medium was replaced with OM for 14 days. The efficiency of transfection was evaluated by qRT-PCR (C) and Western blotting (D). ALP activity was detected by a commercial Alkaline Phosphatase Detection Kit (E). The genes expression of osteogenic marker including Collagen I, Runx2 and Osterix were examined by Western blotting (F) and qRT-PCR (G). Data are expressed as the mean ± SD, n = 3. *P < 0.05.
Mol. Cells 2018;41:575~581
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