Molecules and Cells

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Fig. 2.

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Fig. 2. Schematic diagram of a zinc finger nuclease (ZFN) dimer bound to target DNA.

ZF arrays are fused to the FokI nuclease domain to make a custom nuclease that can recognize unique left and right half-sites. The two ZFNs must bind in an inverted tail-to-tail orientation with their C-termini facing each other. The optimal spacing between the half-sties is 5–7 bp.

Mol. Cells 2017;40:533~541
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