Molecules and Cells

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Fig. 3.

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Fig. 3. STAT3 stabilizes SIAH-1 via a direct interaction. (A) HEK293T cells were transfected with wtSTAT3 and β-catenin. After 24 h, cells were harvested and the mRNA and proteins were extracted for RT-PCR and Western blotting, respectively. (B) HEK293T cells were transfected with siSTAT3 (100 nM) or scrambled siRNA (100 nM, nonspecific siRNA used as a negative control) and with wtSTAT3, β-catenin, or a control pCMV vector. After culturing for 24 h, cells were subjected to Western blotting. (C) HEK293T cells were transfected with wtSTAT3-myc or STAT3-Y705F-myc. After 24 h, an immunoprecipitation assay was performed with an anti-myc-antibody, and Western blotting was completed with the indicated antibodies. (D) HEK293T cells were transfected with wtSTAT3-myc and β-catenin. After 24 h, an immunoprecipitation assay was performed with an anti-myc-antibody and Western blotting was completed with the indicated antibodies.
Mol. Cells 2016;39:821~826 https://doi.org/10.14348/molcells.2016.0212
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