Molecules and Cells

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Fig. 4.

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Fig. 4. miR-217 suppressed 143B cell proliferation, migration, and invasion by targeting KRAS. 100 nM mimic or antagomir was used for cell transfection. Proliferation and migration/invasion assays were performed 24 h post-transfection. Approximately 5000 cells and 5 × 104 cells were used in the cell proliferation assay and migration/invasion assay, respectively. For Western blotting, the total proteins were extracted 48 h after transfection. (A) MiR-217 targeted KRAS in 143B cells. KRAS protein level was downregulated post miR-217 overexpression by mimic, while upregulated post miR-217 knocking down by antagomir. (B) MiR-217 significantly inhibited 143B proliferation. (C, D) MiR-217 significantly suppressed the migration and invasion of 143B cells.
Mol. Cells 2015;38:638~642
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