Molecules and Cells

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 0219-1032   pISSN 1016-8478

Fig. 2.

Download original image
Fig. 2. Sep15 deficiency arrests cells at the G1 phase by downregulating cyclin E1 and E2 expression. (A?B) Sep15 knockdown caused cell cycle arrest at the G0/G1 phase. After the cell cycle was arrested by the treatment with aphidicolin (A) or nocodazole (B), cell cycle analysis was performed as described in Materials and Methods. The numbers 1 and 2 on the bottom of each panel represent the relative DNA contents of the G0/G1 and G2/M phases, respectively. The number of cells and release time after arrest are marked on the left and right side of Y-axis, respectively. Asy designates asynchronized cells. Data shown are representative of at least three independent experiments. (C) Western blot analyses to measure the levels of p21, p27 and cyclin E1 in shSep15+Dox versus -Dox cells and in shSep15/Rescue+Dox versus -Dox cells. (D) Levels of MAD2 in shSep15+Dox versus -Dox cells and in shSep15/Rescue+Dox versus -Dox cells were measured by Western blot analysis.
Mol. Cells 2015;38:457~465
© Mol. Cells